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Basic Hardware Components of HPLC
Solvent Delivery System (Pump) Injector (introduce samples) Column guard Column (containing stationary phase) Dete...
Detectors used in HPLC
MS – provides additional information of molecular weight and structure by fragmentation pattern Photodiode Array (PDA) – Like a U...
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Suitability for a specific application The column must do the job If already using a different column the new column ...
Normal-phase or Liquid-solid Chromatography (LSC)
Normal phase Used polar stationary phase packings and low polarity solvents Non-polar compounds elute out first follow...
Reversed-phase Chromatography (RPC)
Reversed phase Most popular since it can handle the broadest variety of sample types Opposite of normal phase – statio...
Terms used in Chromatography
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Chromatography
Chromatography: Chroma means colour ; technique is used for the separation of different Analytes in sample. This technique is used from m...
Column Packing Materials
Support Type Silica (or alumina) or polymer (cross-linked polystyrene) Bonded groups C18, C8, C4, amino, cyano...
FLAME IONISATION DETECTOR IN GAS CHROMATOGRAPHY
Mass sensitive detector. Operates by mixing hydrogen with carrier gas and burning it at a jet in a stream of air. A ...
Pumps for HPLC
Ideal pumps : 1) Ability to generate high pressure 2) Pulse-free output 3) Accurate control of flow 4) Cor...
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This blog is about chromatography, its technique and about instrument.
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Monday, 23 December 2013
Types of GCMS
1. Time of Flight
2. Quadrupole
3. Ion-trap
4. IRMS
Each of these are applied in different fields
Based on the requirements of analysis and conclusions to be drawn.
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What GCMS users Want ?
Types of GCMS
FLAME IONISATION DETECTOR IN GAS CHROMATOGRAPHY
Detectors used in HPLC
How to choose a column ?
Columns
Pumps for HPLC
Reversed-phase Chromatography (RPC)
Normal-phase or Liquid-solid Chromatography (LSC)
Liquid Chromatographic Modes
Column Packing Materials
Advantages of HPLC over conventional HPLC
Basic Hardware Components of HPLC
Terms used in Chromatography
History of Chromatography
Applications of Uv-Vis Spectrophotometer
Role of sales Person while selling Analytical Inst...
Chromatography
What you mean by Analytical Instruments? Where we ...
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