Reversed-phase Chromatography (RPC)

• Reversed phase
• Most popular since it can handle the broadest variety of sample types
• Opposite of normal phase – stationary phase is non-polar and mobile phase is polar
• C18 columns (e.g. octadecyl, -C₁₈H₃₇)
• Eluents used – aqueous solution of organic solvents e.g. MeOH, ACN, THF, etc.
• Applications
• Amino acids, homologs, herbicides, etc.