Pumps for HPLC

Ideal pumps:

1)      Ability to generate high pressure

2)      Pulse-free output

3)      Accurate control of flow

4)      Corrosion resistant

Role: Deliver the mobile phase

Two groups of pumps:

1)      Constant pressure

2)      Constant volume

Three types of pumps are available:

1)      Reciprocating pumps (90% of Commercial HPLC; produce pulse flow)

2)      Displacement pumps (produce flow that are independent of viscosity and back pressure)

3)    Pneumatic pumps (cannot do gradient and pressure less than 2000psi)

Waters 1525 pump