Normal-phase or Liquid-solid Chromatography (LSC)

• Normal phase
• Used polar stationary phase packings and low polarity solvents
• Non-polar compounds elute out first followed by increasingly polar compounds
• Eluents used – low to moderate polarity e.g. hexane, CH₂Cl₂, CHCl₃, etc.
• Application
• Isomers, compound class separations, etc.
• Ideal for samples with low/moderate polarity, water insoluble and non-ionic