Column Packing Materials

• Support Type
• Silica (or alumina) or polymer (cross-linked polystyrene)
• Bonded groups
• C18, C8, C4, amino, cyano, phenyl
• Diethylaminoethyl (DEAE), sulfonate, quaternary ammonium
• Particle size (d_p): 3-, 5-, 7,- 10- or 20 μm
• Efficiency is inversely proportional to d_p
• Column pressure is inversely proportional to (d_p)²
• Pore size (d_pore): 60-300A
• Wide pore materials (300 A) are used for biomolecules or polymers
• Surface area: 90-400 m²/g
• High surface area maximizes solute interaction with bonded groups