- Structure elucidation of organic
compounds
Location of
peaks and combination of peaks, it can be concluded that
whether the compound is saturated or unsaturated, hetero atoms are present or
not etc.
- Quantitative analysis
- A =
log I0 / It = log 1/ T = – log T =
εbc
- Qualitative analysis
Can
characterize those types of compounds which absorbs UV radiation.
Identification is done by comparing the absorption spectrum with the spectra of
known compounds.
UV absorption spectroscopy is generally used for characterizing aromatic compounds and aromatic olefins.
UV absorption spectroscopy is generally used for characterizing aromatic compounds and aromatic olefins.
- Chemical kinetics
Kinetics of reaction can also be studied using UV spectroscopy. The UV radiation is passed through the reaction cell and the absorbance changes can be observed. - Quantitative analysis of
pharmaceutical substances
Many drugs are either in the form of raw material or in the form of formulation. They can be assayed by making a suitable solution of the drug in a solvent and measuring the absorbance at specific wavelength. - Molecular weight
determination
Molecular weights of compounds can be measured spectrophotometrically by preparing the suitable derivatives of these compounds. - As HPLC detector
A UV/Vis
spectrophotometer may be used as a detector for HPLC. The presence of an
analyte gives a response which can be assumed to be proportional to the
concentration.
- Detection of Impurities
Perkin Elmer Uv-Vis
0 comments:
Post a Comment