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Advantages of HPLC over conventional HPLC
Rapid and precise quantitative analysis Typical analysis time of 5-20 min, precision <0.5-1% RSD Automated analysis...
Liquid Chromatographic Modes
Normal-phase or Liquid-solid (NP, LSC) Separation based on adsorption/desorption of the analyte onto a polar surface (s...
Reversed-phase Chromatography (RPC)
Reversed phase Most popular since it can handle the broadest variety of sample types Opposite of normal phase – statio...
Pumps for HPLC
Ideal pumps : 1) Ability to generate high pressure 2) Pulse-free output 3) Accurate control of flow 4) Cor...
Applications of Uv-Vis Spectrophotometer
Structure elucidation of organic compounds Location of peaks and combination of peaks, it can be concluded that whether the c...
Terms used in Chromatography
Analyte : The substance / compound to be separated Preparative chromatography : Purify sufficient quantities of a substance ...
Column Packing Materials
Support Type Silica (or alumina) or polymer (cross-linked polystyrene) Bonded groups C18, C8, C4, amino, cyano...
FLAME IONISATION DETECTOR IN GAS CHROMATOGRAPHY
Mass sensitive detector. Operates by mixing hydrogen with carrier gas and burning it at a jet in a stream of air. A ...
Chromatography
Chromatography: Chroma means colour ; technique is used for the separation of different Analytes in sample. This technique is used from m...
Types of GCMS
1. Time of Flight 2. Quadrupole 3. Ion-trap 4. IRMS Each of these are applied in differe...
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This blog is about chromatography, its technique and about instrument.
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Saturday, 21 December 2013
Basic Hardware Components of HPLC
Solvent Delivery System (Pump)
Injector (introduce samples)
Column guard
Column (containing stationary phase)
Detectors (“eyes” )
Waste Collector
Recorder (Data Collection)
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What GCMS users Want ?
Types of GCMS
FLAME IONISATION DETECTOR IN GAS CHROMATOGRAPHY
Detectors used in HPLC
How to choose a column ?
Columns
Pumps for HPLC
Reversed-phase Chromatography (RPC)
Normal-phase or Liquid-solid Chromatography (LSC)
Liquid Chromatographic Modes
Column Packing Materials
Advantages of HPLC over conventional HPLC
Basic Hardware Components of HPLC
Terms used in Chromatography
History of Chromatography
Applications of Uv-Vis Spectrophotometer
Role of sales Person while selling Analytical Inst...
Chromatography
What you mean by Analytical Instruments? Where we ...
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