Terms used in Chromatography

• Analyte : The substance / compound to be separated
• Preparative chromatography :  Purify sufficient quantities of a substance for further use, rather than analysis.
• Analytical chromatography is used to determine the existence and the concentration of analyte(s) in a sample.
• A bonded phase is a stationary phase that is covalently bonded to the support particles or to the inside wall of the column tubing.
• A chromatogram is the visual output of the chromatograph. In the case of an optimal separation, different peaks or patterns on the chromatogram correspond to different components of the separated mixture
• Stationary Phase : The immobilized phase that is on the support particles or the inner walls of the column tubing
• Mobile phase : The phase that moves through the stationary phase in a definite direction and at a defined speed. Liquid – LC, CE, Gas in GC, SFC.